Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system.

Author's response to reviews Title: Identification of differentially expressed genes using annealing control primer system in stage III serous ovarian carcinoma Authors:

Annealing control primer system for identification of differentially expressed genes on agarose gels.

We developed GeneFishing technology, an improved method for the identification of differentially expressed genes (DEGs) using our novel annealing control primer (ACP) system. Because of high annealing specificity during PCR using the ACP system, the application of the ACP to DEG discovery generates reproducible, authentic, and long (100 bp to 2 kb) PCR products that are detectable on agarose ge...

Identification of differentially expressed genes in murine embryos at the blastocyst stage using annealing control primer system.

The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method that involves an...

Identification of drought induced differentially expressed genes in barley leaves using the annealing control-primer-based GeneFishing technique

In the present study, we have used an annealing-control-primer (ACP)-based differential display reverse transcription-polymerase chain reaction (RT-PCR) method to identify drought-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 12 up-regulated and 3 down-regulated genes were identified and sequenced. Temporal expression patterns of the 15 upor d...

Identification of differentially regulated genes in bovine blastocysts using an annealing control primer system.

The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) technology that involve...